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Alexander Biddle (April 29, 1819 – May 2, 1899) was an officer in the Union Army during the American Civil War.

The fourth child of Thomas and Christine Biddle, he was educated in Pennsylvania, enrolling at the University of Pennsylvania in 1834 and graduating in 1838. In 1842, he joined his fathers company, where he worked until the beginning of the Civil War.

In 1849, he joined the first troop of the Philadelphia City Cavalary in which he served until 1858. In 1855 he married Julia Williams Rush, then on September 1, 1862, he joined the 121st Pennsylvania Volunteer Infantry, serving under Chapman Biddle, with whom he fought at the Battle of Fredericksburg, the Battle of Chancellorsville, the Battle of Gettysburg (under Abner Doubleday) and the Battle of Bristoe Station.

After the war he retired from Thomas Biddle & Co., and in 1874 he was named a director of the Pennsylvania Railroad. He also served as a director for the Philadelphia Savings Fund Company and the Lehigh Navigation Company among others, and as executor of the estate of James Rush, was instrumental in the erection of the Ridgway Library (part of the Library Company of Philadelphia).

His name is inscribed on the 121st Pennsylvania's monument along South Reynolds Avenue in the Gettysburg National Military Park.

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