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The 5th Regiment, Vermont Volunteer Infantry (or 5th VVI) was a three years' infantry regiment in the Union Army during the American Civil War. It served in the Eastern Theater, predominantly in the VI Corps, Army of the Potomac, from September 1861 to June 1865. It was a member of the Vermont Brigade.

The regiment was mustered into Federal service on September 16, 1861, at St. Albans, Vermont. It was engaged in, or present at, Lee's Mill, Williamsburg, Golding's Farm, Savage's Station and White Oak Swamp during the Peninsula campaign; Crampton's Gap and Antietam during the 1862 Maryland campaign; Fredericksburg, Marye's Heights, and Salem Church; Gettysburg and Funkstown during the Gettysburg campaign; Rappahannock Sation; the Wilderness, Spotsylvania, and Cold Harbor during the Overland Campaign; Charlestown, Opequon, Winchester, Fisher's Hill, and Cedar Creek during the Shenandoah Valley campaign and in the siege of Petersburg.

The regiment lost during service: 201 men killed and mortally wounded, 4 died from accident, 1 executed, 21 died in Confederate prisons and 112 died from disease; total loss: 339.

The regiment mustered out of service on June 29, 1865.